Application of membrane processes for archaea isolation / Michał Polus, Anna M. Anielak, Michał Zielina, Elżbieta Ulman // W: Membranes and membrane processes in environmental protection / ed. by Krystyna ... więcej

Application of membrane processes for archaea isolation / Michał Polus, Anna M. Anielak, Michał Zielina, Elżbieta Ulman // W: Membranes and membrane processes in environmental protection / ed. by Krystyna Konieczny, Irena Korus. – Warsaw ; Gliwice : Environmental Engineering Committee Polish Academy of Sciences, 2014. – (Monografie / Polska Akademia Nauk. Komitet Inżynierii Środowiska ; 118). – S. 381-391 : il. – Bibliogr. 24 poz., Abstr., Streszcz. – Tyt. streszcz.: Zastosowanie procesów membranowych do izolacji archeanów. – ISBN 978-83-63714-17-8

In environmental engineering the membrane processes are used mainly for water and wastewater treatment. From the solutions various impurities are removed, depending on the type of membrane and the actual ... więcej

In environmental engineering the membrane processes are used mainly for water and wastewater treatment. From the solutions various impurities are removed, depending on the type of membrane and the actual process employed. The authors adopted the ultrafiltration process, acting as a micron sieve, for isolation of the little-known Archaea microorganisms, leached from an organic material. The isolation was performed using MCE membranes of different porosities. For each fraction, DNA isolation was carried out with the GeneMATRIX Soil DNA Purification Kit (EURx, Poland). Analysis of the presence of DNA material in the individual fractions revealed that the whole group of ammonia oxidizing Archaea microorganisms fits within the top range, i.e.> 3.0 m. At the same time, analysis of the size and number of particles in the different fractions showed that the test material was very diverse, and microorganisms heavily contaminated by other substances