Spectroscopic studies of dual fluorescence in 2-(4-fluorophenylamino)-5-(2,4-dihydroxybenzeno)-1,3,4-thiadiazole: effect of molecular aggregation in a micellar system [Poster] / A. Matwijczuk, D. Karcz, ... więcej

Spectroscopic studies of dual fluorescence in 2-(4-fluorophenylamino)-5-(2,4-dihydroxybenzeno)-1,3,4-thiadiazole: effect of molecular aggregation in a micellar system [Poster] / A. Matwijczuk, D. Karcz, A. Matwijczuk, A. Górecki, A. Niemczynowicz, R. Walkowiak, L. Adwent, A. Szcześ, E. Chruściel, A. Niewiadomy // European Biophysics Journal with Biophysics Letters [Dokument elektroniczny]. – 2019, Vol. 48, Suppl. 1, s. 212. – Tryb dostępu: https://link.springer.com/content/pdf/10.1007%2Fs00249-019-01373-4.pdf. – Oznaczenie ref./art.: P-541. – Mat. konf.: JOINT 12th EBSA congress and 10th ICBP – IUPAP congress, Madrid, Spain, 20-24.06.2019. – doi: 10.1007/s00249-019-01373-4. – ISSN 1432-1017

This work reports on spectroscopic investigation into a selected 1,3,4thadiazole derivative from the series of 2-(4-fluorophenylamino)-5-(2,4dihydroxybenzeno)-1,3,4-thiadiazole (FABT). The experiments ... więcej

This work reports on spectroscopic investigation into a selected 1,3,4thadiazole derivative from the series of 2-(4-fluorophenylamino)-5-(2,4dihydroxybenzeno)-1,3,4-thiadiazole (FABT). The experiments consisted on preparation of a micellar system with use of a non-ionic detergent Triton X100 and the subsequent spectroscopic measurements of the micelles formed. The steady state fluorescence revealed a dual fluorescence phenomenon emerging from the particular molecular organisation of the compound, which in turn depends both on the concentration of the detergent and the concentration of the compound itself. Dual fluorescence emission in FABT micelles was observed in methanol-water mixture, wherein the dual fluorescence of this compound had never been reported before. It has been noted that the occurrence of this effect was related to the process of molecular aggregation taking place between FABT molecules in the micellar system in question. This hypothesis was evidenced based on a series of spectroscopic experiments, namely the UV-Vis electronic absorption, resonance light scattering (RLS), emission and excitation fluorescence spectra, as well as measurements of dynamic light scattering (DLS) and Principal Component Analysis (PCA). Moreover, the measurements carried out at various temperatures demonstrated a reversibility the effects observed. Results obtained from steady state fluorescence spectra together with those from time-correlated single photon counting (TCSPC) measurements suggest that dual fluorescence occurs at detergent concentrations necessary to form micelles, facilitating the aggregation of FABT molecules. The comparison of fluorescence effects occurring in methanol-water mixture to those obtained from previous measurements performed on various FABT analogues suggests the possibility of charge transfer (CT) within the range of detergent concentrations at which the aforementioned fluorescence effects are observed.